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Modifications in mechanical design of the coherence controlled holographic microscope
Matela, Milan ; Šerý, Mojmír (referee) ; Kolman, Pavel (advisor)
The aim of diploma thesis are modifications in mechanical design of the coherence controlled holographic microscope which will lead to reduction of some deficiencies. In this thesis there is a brief description of the history interference microscopy and differences between each types of interference microscopes. In this work there are stated some design deficiencies of actual condition of the microscope and also several solutions are suggested and shortly described. Then the best solution is chosen which is adjusted to a final form. It is described in detail and then realized. There is a summary included in the conclusion whether the new solution is suitable and if it fulfills the required condition.
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Analysis of changes in the phenotype of tumour cells induced by migrastatics in quantitative phase imaging
Kolínková, Veronika ; Netíková,, Irena Štenglová (referee) ; Veselý, Pavel (advisor)
This thesis focuses on the observation of living cells using the non-invasive methods of quantitative phase imaging (hiQPI). The imaging is enabled by a coherence-controlled holographic microscope (CCHM) developed in the Laboratory of Experimental Biophotonics at the VUT. Using this imaging technology, morphological changes of A549 and MCF7 after application of potential migrastatic drugs tumor cells are evaluated in the experimental part of the thesis. Migrastatics are defined as already approved drugs that could prevent the migration of cancer cells from the primary tumor and thus prevent metastasis. The RAC-GM (Rapid Assessment of Cell Growth and Migration in Vitro) method was chosen to assess their effect on tumor cells.
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Design of the imaging part of the coherence-controlled holographic microscope
Dvořák, Vladislav ; Dostál, Zbyněk (referee) ; Špaček, Matěj (advisor)
The Experimental Biophotonics Research group at Brno University of Technology has been involved in the development of Coherence controlled holographic microscope (CCHM). For the purposes of further development in this field, it is necessary to build an open laboratory set-up of the microscope, which has only been available in a commercial design. The objective of this thesis is to design and assemble its imaging unit. The first part of the thesis is dedicated to a general overview of microscopic methods, with an emphasis on interference microscopy and principle of CCHM microscope. In the second part, different optical set-up concepts are discussed and compared. Then, the optical components are selected and the unknown parameters of these components are experimentally measured. With the use of measured parameters, the optical tracing and mechanical design are introduced. The imaging unit has been assembled based on the suggested design and experimentally verified.
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Analysis of changes in the phenotype of tumour cells induced by migrastatics in quantitative phase imaging
Kolínková, Veronika ; Netíková,, Irena Štenglová (referee) ; Veselý, Pavel (advisor)
This thesis focuses on the observation of living cells using the non-invasive methods of quantitative phase imaging (hiQPI). The imaging is enabled by a coherence-controlled holographic microscope (CCHM) developed in the Laboratory of Experimental Biophotonics at the VUT. Using this imaging technology, morphological changes of A549 and MCF7 after application of potential migrastatic drugs tumor cells are evaluated in the experimental part of the thesis. Migrastatics are defined as already approved drugs that could prevent the migration of cancer cells from the primary tumor and thus prevent metastasis. The RAC-GM (Rapid Assessment of Cell Growth and Migration in Vitro) method was chosen to assess their effect on tumor cells.
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Modifications in mechanical design of the coherence controlled holographic microscope
Matela, Milan ; Šerý, Mojmír (referee) ; Kolman, Pavel (advisor)
The aim of diploma thesis are modifications in mechanical design of the coherence controlled holographic microscope which will lead to reduction of some deficiencies. In this thesis there is a brief description of the history interference microscopy and differences between each types of interference microscopes. In this work there are stated some design deficiencies of actual condition of the microscope and also several solutions are suggested and shortly described. Then the best solution is chosen which is adjusted to a final form. It is described in detail and then realized. There is a summary included in the conclusion whether the new solution is suitable and if it fulfills the required condition.
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